个人简介
郭宵，博士，学术副教授，硕士生导师，长期从事多糖降解酶的资源挖掘、酶学表征、理性设计与分子机制研究。围绕生物质酶解转化，建立了从环境微生物筛选、基因组CAZyme注释到候选基因挖掘的完整体系；完成多个家族酶种的异源表达、纯化及酶学性质表征，在底物谱分析、环境因子响应及产物检测等方面经验丰富。综合运用结构预测、分子对接和分子动力学模拟，结合关键位点突变验证，揭示催化机制与底物选择性规律。主持多项省厅级项目，发表多篇高水平论文。承担《微生物学》《疫苗工程》《微生物学实验》《生物制药专业综合实验》等教学工作。
研究方向
基因挖掘与克隆表达、酶的理性设计及分子机制探究、蛋白表达调控与作用机制研究等。
承担项目
[1] 山东科技大学“菁英计划”B类人才引进项目，30万，主持.
[2] 山东省自然科学基金面上项目, ZR2024MC158, 提高生物质转化酶LPMO-R17L/D20G的热稳定性及分子机制研究, 2025.01.01-2027.12.30, 10万元, 主持.
[3] 山东省自然科学基金青年项目, ZR2022QC199, 裂解性多糖单加氧酶的理性设计及表达调控研究, 2023.01.01-2025.12.30, 15万元, 主持.
[4] 青岛市博士后应用研究项目, 01020240116, 转化秸秆为生物能源的酶制剂的分子改造及应用, 2021.07-2023.06, 5万元, 主持.
[5] 工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室(天津科技大学), 开放课题项目, 裂解性多糖单加氧酶的理性设计及其高效表达工业菌株的构建, 2022.01-2023.01, 2万元, 主持.
个人成果
[1] Xiao Guo, Fei Li, Bo Wang*, Fufeng Liu*. Plant-based nanomaterials for sustainable applications in food, medicine, and environmental remediation: A review, International Journal of Biological Macromolecules, 2025, 332, 148605. (一区Top, IF=8.025)
[2] Xiao Guo, Xinming Wu, Bo Wang* and Fufeng Liu*. “Biopulping, Biobleaching, and Deinking.” Forest Microbiology: Volume 4 - Biotechnology Applications in Forestry, edited by Fred O. Asiegbu and Andriy Kovalchu, Elsevier, 2025: 307-322. (Book)
[3] Xiao Guo, Yajing An, Fuping Lu, Fufeng Liu*, Bo Wang*. Efficient secretory production of lytic polysaccharide monooxygenase BaLPMO10 and its application in plant biomass conversion, International Journal of Molecular Sciences, 2023, 24(11): 9710. (二区top, IF=6.208)
[4] Xiao Guo, Luying Jiang, Yajing An, Fuping Lu, Fufeng Liu*, Bo Wang*. Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability, Enzyme and Microbial Technology, 2023, 168: 110255. (三区, IF=3.705)
[5] Xiao Guo, Yajing An, Fufeng Liu, Fuping Lu, Bo Wang*. Lytic polysaccharide monooxygenase - a new driving force for lignocellulosic biomass degradation, Bioresource Technology, 2022, 362: 127803. (一区Top, IF=11.889)
[6] Xiao Guo, Yajing An, Fuping Lu*, Fufeng Liu*. Optimization of synergistic degradation of steam exploded corn straw by lytic polysaccharide monooxygenase R17L and cellulase, Industrial Crops and Products, 2022, 182: 114924. (一区top, IF=6.449)
[7] Xiao Guo, Yajing An, Luying Jiang, Jiayu Zhang, Fuping Lu*, Fufeng Liu*. The discovery and enzymatic characterization of a novel AA10 LPMO from Bacillus amyloliquefaciens with dual substrate specificity, International Journal of Biological Macromolecules, 2022, 203: 457-465. (一区Top, IF=8.025)
[8] Xiao Guo, Chengcheng Chai, Yajing An, Chong Peng, Nian Shi, Wenqian Wang, Fuping Lu, Yujie Dai*, Fufeng Liu*. Rational design of signal peptides for improved MtC1LPMO production in Bacillus amyloliquefaciens, International Journal of Biological Macromolecules, 2021, 175: 262-269. (一区Top, IF=8.025)
[9] Xiao Guo, Yajing An, Chengcheng Chai, Jingcheng Sang, Luying Jiang, Fuping Lu, Yujie Dai, Fufeng Liu*. Construction of the R17L mutant of MtC1LPMO for improved lignocellulosic biomass conversion by rational point mutation and investigation of the mechanism by molecular dynamics simulations, Bioresource Technology, 2020, 317: 124024. (一区Top, IF=11.889)
[10] Xiao Guo, Jingcheng Sang, Chengcheng Chai, Yajing An, Zhifeng Wei, Huitu Zhang, Lijuan Ma, Yujie Dai*, Fuping Lu*, Fufeng Liu*. A lytic polysaccharide monooxygenase from Myceliophthora thermophila C1 and its characterization in cleavage of glycosidic chain of cellulose, Biochemical Engineering Journal, 2020, 165(15): 107712. (二区, IF=4.446)
招生专业：生物学
邮 箱：guoxiao@sdust.edu.cn
